CCAAT enhancer binding protein α suppresses proliferation, metastasis, and epithelial-mesenchymal transition of ovarian cancer cells via suppressing the Wnt/β-catenin signaling

CCAAT enhancer binding protein alpha (CEBPA, also known as C/EBPα) is a transcription factor that plays an essential role in regulating terminal differentiation and cell proliferation of many tissues. The objective this study to explore the potential function CEBPA ovarian cancer. expression cancer samples adjacent normal tissues was evaluated by qRT-PCR. putative cells immunohistochemistry, western blot, viability assay, BrdU incorporation soft agar colony formation transwell migration invasion tumor xenograft formation, lung metastasis model. We found downregulated predicted poor prognosis. CRISPR/Cas9-mediated knockout promoted proliferation, anchorage-independent growth, migration, invasion, EMT cells, while enforced CEPBA suppressed EMT, cells. Furthermore, we activated Wnt/β-catenin signaling overexpression activation. Our data indicated acted suppressor cancer, might be prognostic marker for treatment.